This lesson is still being designed and assembled (Pre-Alpha version)

This lesson is part of The Carpentries Incubator, a place to share and use each other's Carpentries-style lessons. This lesson has not been reviewed by and is not endorsed by The Carpentries.

RNA-seq analysis with Bioconductor

FIXME: This lesson introduces the learner to RNA-seq analysis with Bioconductor.

Prerequisites

Familiarity with R, for example corresponding to the ‘Introduction to R’ module.

Familiarity with statistical hypothesis testing (e.g., t-tests) and p-values.

Familiarity with the biological basics of gene expression and RNA sequencing.

Schedule

	Setup	Download files required for the lesson
00:00	1. Introduction and setup
00:00	2. Introduction to RNA-seq	What is RNA-seq?
00:00	3. Experimental design	How do we design experiments optimally? How do we interpret a given design?
00:00	4. Importing and annotating quantified data into R	How do we get our data into R?
00:00	5. Exploratory analysis and quality control
00:00	6. Differential expression analysis	How do we find differentially expressed genes?
00:00	7. Gene set analysis	How do we find differentially expressed pathways?
00:00	Finish

The actual schedule may vary slightly depending on the topics and exercises chosen by the instructor.